In this tutorial, we will use a sample data gathered during a clinical trial of a new chemical/pesticide on tobacco Budworms. The subjects (i.e. Budworms) are grouped into batches of 20, and exposed to different doses of the chemical. The results are summarized below
Our objective here is to model (and forecast) the effectiveness of the new chemical using different dosages, and explain, to some extent, any variation based on the gender of the budworm. Furthermore, we want to express the results in term of the worm mortality rates (i.e. probability).
We plot the data into two separate curves: males and females. It is apparent that mortality rate is affected by those two factors: gender and dosage.
We will make two assumptions: (1) the results for each trial (i.e. batch) are drawn from a Binomial distributed population; we would like to estimate p - the probability of success (i.e. worm’s death). The probability (p) is allowed to vary across different trials (batches). (2) The probability of success is affected by two factors: gender of the subject and administered dosage of the drug.
Based on these two assumptions, we would model this relationship:
We are ready now to propose a statistical model: generalized linear model in Excel with residuals following the Binomial distribution.
For now, we choose “Logit” as our link (transform) function, specify the trial or batch size(20), and instruct the Wizard to calibrate (i.e. compute optimal values for the coefficients). Leave the Goodness-of-fit and residual diagnosis options checked.
In this case, the Generalized Linear Model in Excel (GLM) Wizard has calibrated the model’s coefficients, so we can skip this step.
But, in the event we wish to experiment with different link functions: LOGIT, PROBIT or LOG-LOG, then we need to re-calibrate the model. To do so, we can either:
- Create a new model with the wizard, or,
- (2) Change the “Lvk” parameter in an existing model table, and run the calibration using NumXL toolbar
Step 1: Select the cell that acts as a header for the model table
Step 2: Click on the calibration icon/menu (Excel 2003)
Step 3: Click on “Solve” button in the Solver window
Once the model is calibrated, and we are happy with the residuals, we can use it to construct our forecast mean (and confidence interval around it).
Using NumXL function (GLM_FORE), we can compute the mean. Using GLM_FORECI, we can compute the upper and lower limit of the confidence interval.
Plotting the data again (actual) versus the model values.
The dots represent the sample data, while the center line is the forecast mean. The shaded regions in the graphs are the 95% confidence intervals.
- The forecast error decrease as we increase the dosage (C.I. gets tighter). This is evident in male and female batches
- The logarithmic relation detected when we plot the raw data can be merely a data anomaly; the Generalized Linear Model in Excel shows more like a quadratic-type of relationship.
- The mean is not exactly the center of the confidence interval due to the discrete-nature of the underlying binomial distribution, and the small batch/trial size.